Description

Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.

Langue

en

Version

Broché

Date de sortie initiale

23 août 2016

Nombre de pages

425

Illustrations

Avec illustrations

Personnes impliquées

Auteur principal

Ronald P. van Rij

Rédacteur en chef

Ronald P. van Rij

Editeur principal

Humana Press Inc.

Informations sur le fabricant

Nom du fabricant

Springer Nature Customer Service Center GmbH

Adresse du fabricant

– 69115 – – DE

Adresse électronique du fabricant

ProductSafety@springernature.com

Informations sur le fabricant

Les informations du fabricant ne sont actuellement pas disponibles

Autres spécifications

Hauteur de l’emballage

254 mm

Largeur d’emballage

178 mm

Largeur du produit

178 mm

Livre d‘étude

Non

Longueur d’emballage

254 mm

Longueur du produit

254 mm

Poids de l’emballage

1097 g

Police de caractères extra large

Non

Édition

Softcover reprint of the original 1st ed. 2011

EAN

EAN

9781493958252

Sécurité des produits

Opérateur économique responsable dans l’UE

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Médecine et Soins infirmiers

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Microbiologie

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Langue

Anglais

Type de livre

Paperback

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